Science International  Volume 1 Issue 5, 2013

Research Article

Appraisal of Eighteen Silkworm (Bombyx mori L.) Genotypes Using Pre and Post Cocoon Characters
Ebrahim Talebi
Darab Branch, Islamic Azad University, Darab, Fars, Iran

Shivakumar Bakkappa
DOS in Sericulture Science, University of Mysore, Manasagangotri, 570 006, Mysore, India

ABSTRACT:
This study was done on simultaneously reared silkworm genotypes over four consecutive years (2004-2007) and at different seasons. Eighteen silkworm genotypes were tested in the study from bivoltine, multivoltine and mutants from the Germplasm Bank of DOS in Sericulture Science, University of Mysore, Mysore. Analysis of variance was done for nine quantitative pre and post cocoon characters in eighteen silkworm genotypes, results clearly showed that all nine economic traits exhibited highly significant difference. The highest record for mean hatching percentage was from the G111 genotype at 98.51% and the MU1 race revealed the least mean value of 98.02%.Genotypes MG414 (3.79 g) and PM (661 h) exhibited maximum evaluations for larval weight and larval duration, respectively. Results of this investigation provide information on morphological dissimilarity among silkworm strains calculated from the generalized distance, which is an extension of the Euclidian distance. The dendrogram UPGMA, produced from the cluster analysis, demonstrated two distinct clusters, divided in terms of having genetic distances 0.45 to 3.27 from the respective clusters and the genetic architecture of traits is used as a key for evolutionary geneticists.
 
    How to Cite:
Ebrahim Talebi and Shivakumar Bakkappa , 2013. Appraisal of Eighteen Silkworm (Bombyx mori L.) Genotypes Using Pre and Post Cocoon Characters. Science International, 1: 118-123
DOI: 10.5567/sciintl.2013.118.123
 


INTRODUCTION
The silkworm is not only a commercially important insect but identification of its genetic characters is also an important laboratory tool for scientific breeding. This type of genetic analysis has been applied during the last few decades to enable animal and plant breeders to increase the productivity of commercial plants and animals. It has been estimated that, more than 3000 silkworm stains are available worldwide due to various ongoing breeding programs1,2. These univoltine and bivoltine silkworm varieties are qualitatively and quantitatively superior races, whereas multivoltine are relatively inferior in terms of traits but superior in terms of survival rate and hardiness. However, in tropical countries like India, multivoltine silkworm strains are used for silk production, as they are well adapted to tropical climates.

During the process of breeding, the basic principles of genetics are applied to understand patterns of inheritance that determine many of qualitative and quantitative characters, successfully applied to increase livestock production. It is imperative to know that in the silkworm Bombyx mori, quality and quantity of the silk produced are determined by the interaction between the genetic potential of silkworm races and the specific environment to which they are exposed. An extensive study is needed to improve existing strains for commercial purposes and to develop new strains through breeding programmes aimed at improving silk productivity, adaptability to a local environment and disease resistance and/or tolerance3,4.

The primary goal of silkworm breeding programmes is the simultaneous genetic improvement of multiple traits in general and the superiority of silkworm varieties is judged according to manifestations of traits in any selection programme. Normally breeders deal with more than one character, either directly or indirectly but a common index could be used to evaluate multiple traits in order to judge the superiority of a variety impartially. In order to maintain these genetic resources for heterosis, classification and characterization of these strains is very important. In this study, eighteen Bombyx mori strains were evaluated for nine quantitatively important characters and for feasibility of using these strains in breeding programs.


MATERIALS AND METHODS
This study was done on the simultaneous rearing, over about four years (2004-2007) in different seasons of the year, using eighteen different genotypes trains of bivoltine, multivoltine and mutant varieties drawn from the Germplasm Bank of DOS in Sericulture Science, University of Mysore, Mysore. In this study, 18 silkworm strains were evaluated for nine quantitatively important traits to determine their feasibility for use in breeding programmes providing data that will be useful in genetic and breeding programmes for the production of superior silkworm strains and hybrids. After incubation of eggs at 25±10°C and relative humidity of 80±5%, the block box was taken out on the 8th day to achieve uniformity in hatching. Rearing was conducted in three seasons viz., pre-monsoon, monsoon and post-monsoon and larvae hatched from each laying were reared separately under uniform laboratory conditions as described by Yokoyama5 and Krishnaswami6.

The variance components were estimated using SAS and Minitab software7. The statistical models for estimates of each trait were:

Yijk = μ+Gi+Yj+ (GY)ij+eijk

Where:

μ = Population mean
Gi = ith Genotype effect
Yj = jth year effect
(GY)ij = ith Genotype and jth year instruction effect
eijk = ijkth Error


RESULTS AND DISCUSSION
Table 1 shows the analysis of variance (mean square values) for nine quantitative pre and post cocoon characters in eighteen silkworm genotypes. The Table clearly demonstrates that all the nine economic traits exhibited significant difference at 0.01 level of probability. The interaction data of ANOVA shows that none of the traits revealed significant difference except for that of larval weight at 5.02, exhibiting highly significant difference.

Furthermore, characteristics of nine traits in eighteen different voltine groups including indigenous and exotic genotypes were analyzed in this study to determine levels of fecundity in terms of hatching percentage, larval weight, larval duration, yield by number/10,000 larvae brushed (YBN), yield by weight/10,000 larvae brushed (YBW), cocoon weight, shell weight and shell percentage: characteristics that have an important role in the selection of specialized strains and are related to silkworm cocoon and silk yarn production. During the larval stage, the silkworm accumulates energy reserves for cocoon production and for the last stage of its life cycle. The mean values of nine pre and post cocoon traits of eighteen selected genotypes (Table 2) show different quantitative traits’ variations in selected races among which, the MG414 and C. nichi races exhibited mean values for maximum and minimum fecundity at 571.47 and 437.42, respectively. Whereas in the case of hatching percentage, the mean value of G111 genotype at 98.51% was the highest and the MU1 race revealed the least mean value of 98.02%. However, only the strains MG414 (3.79 g) and PM (661 h) exhibited maximum levels of larval weight and larval duration, respectively. The C. nichi race recorded the lowest amount for traits of larval weight and larval duration. However, these parameters were indicated as correlates to predict silkworm silk production capacity8.

Furthermore, this Table demonstrates that by extending the parameters of YBN and YBW the highest evaluations of 9387.70 No. and 13.25 kg in C. nichi and MU406, respectively in the same traits the least being recorded by Knobbed (8603.50 No.) and Ursa (7.62 g) so far. However, it is very important to glance over the C. nichi race and Ursa mutant that exhibited the lowest of three post cocoon parameters (CW, SW and SR) at records of 0.90, 0.10 and 11.37 g), respectively, whereas, the highest records for CW and SW traits were scored by MU405 and MU406 at constant 1.49 and 0.27 g in both races, respectively but the maximum SR was recorded in MU406 race at 18.33%. The most important traits to correlate with silk production performance of the silkworm are those of cocoon weight, shell weight and shell percentage. An initial analysis of all strains showed that the mean value of cocoon weight, shell weight and shell percentage of the MU405, MU406 and MG414 indigenous races were superior and that the lowest values were recorded for C. nichi Chinese races and Ursa Japanese mutants are from exotic genotypes. Therefore, the shell ratio measure is an estimate that involves cocoon weight and shell weight traits simultaneously with other factors and defines the cocoon’s final value. Furthermore, in general other races/mutants, although small differences among them were observed, they were not significant.

Shell weight and silk gland weight are traits that determine the potential for silk production because the energy for silk formation is contained within this gland8.

Table 1: Analysis of nine quantitative characters of eighteen silkworm genotypes in different years

Table 2: Analysis of pre and post-coccon traits of eighteen silkworm genotypes

Table 3: Analysis of silkworm nine important larval traits in four different years

Evaluations for MU406 MU405 showed cocoon weights 1.49, 1.49 shell weights 0.27, 0.27 and shell percentages of 18.33 and 18.05, respectively; mean values obtained for all eighteen strains were higher than those recorded in other studies in the available literature8,9,10. Evaluations for cocoon weight, shell weight and shell percentage are very important silkworm parameters in terms of economic viability, they are highly inheritable, affected by the additive gene action and they allow a better response to the selection process11,12.

However, mean values of nine economic traits in four different years (Table 3) show that, the fecundity trait was highest (506) in the year 2006 and much less in 2005 (470) followed by rates of 487 and 494 in the years 2004 and 2007, respectively. But in these years the trait of hatching percentage exhibited 98.43 and 97.96 maximum and minimum percentages in the years 2004 and 2007, respectively and moderate traits recorded for the remaining two years were 2005 (98.31) and (98.39). Furthermore, in this Table, evaluations for larval weight, larval duration and YBN recorded the highest levels of 30.14 g, 592.09 h and 9151.50 No. in the same year (2004) and in 2007 all three traits showed the least mean values of 26.26 g, 532.61 h and 8964 No. and the remaining two years showed moderate mean values, respectively. Evaluations for YBW were highest in 2006 (10.70) and least in 2007 (992) followed by moderate in the other two years. However, evaluations for CW, SW and SR were very high 1.25, 0.20 and 15.94 in the same year 2006 but records for the year 2007 were CW (1.16) and SW (0.18) except that SR exhibited in 2004 was 15.34.

Figure 1 clearly showed the comparisons analysis between nine quantitative traits of all eighteen races. In this study, information on morphological dissimilarity among the silkworm strains of the Germplasm Bank was calculated from the generalized distance of Mahalanobis13 which is an extension of the Euclidian distance. However, the dendrogram produced is that of an unweighted pair group method with arithmetic means (UPGMA) and through cluster analysis (Fig. 2); results demonstrate a clear division into two huge clusters (groups) on the basis of genetic distance of 0.45 to 3.27 from the respective two clusters and the genetic architecture of these traits is thus a key concern to evolutionary geneticists. These grouping methods allowed for the subdivision of an original group of observations into several subgroups in such a way that homogeneity was obtained inside the subgroups and heterogeneity among the subgroups. The method employed for grouping in terms of UPGMA uses the average distance among all the equal genotypes for the formation of each group14,15.

Figure 1(a-c): Comparison between nine quantitative traits of Bivoltine, Multivoltine and Mutant (Bombyx mori L.)

Figure 2: Dendrogram showing the relationships among eighteen silkworm genotypes, 1: C. nichi, 2: G111,, 3: G112, 4: GNP, 5: Hosa Mysore, 6: Kolar gold, 7: Knobbed, 8: MG414, 9: MU1, 10: MU405, 11: MU406, 12: Nistari, 13: Pure Mysore, 14: Tamil Nadu white, 15: Ursa, 16: White eyes mutant, 17: Yellow mutant and 18: Zebra

However, strains such as Ursa, Zebra, Knobbed, Yellow Mutant, G111 and G112 were clustered in one group and the remaining genotypes were grouped together and formed another cluster. Furthermore, these two clusters are divided into several sub-groups (sub-clusters), whereby Ursa and Zebra mutants fall in one sub-group and another Knobbed, Yellow Mutant, G111 and G112 fall together in another sub-group.

However, in the case of other cluster races such as GNP, MU1, HM, TNW, Kolar Gold, Nistari and White Eyes Mutant, they fall in to one sub-group and MG414, MU406 and MU405 races fall together to form another sub-group and ultimately PM and C. nichi races were interlinked together to the previous sub-groups, respectively (Table 4).

Figure 3 indicates different distances between all genotypes. Results of this research confirm that optimum difference between genotypes might be used for prediction and even to obtain heterosis in a silkworm-breeding programme15,16.

Table 4: Standardized variables amalgamation steps, Euclidean distance and single linkage of eighteen silkworm genotypes

Figure 3: Two-dimensional display of distance between eighteen silkworm genotypes based on nine quantitative traits

Arunachalam16 reported that there is an optimum level of genetic divergence between parents to obtain heterosis in the F1 generation and that they reported that it may not be logical to advocate the use of extremely divergent parents to obtain heterotic combinations. In this investigation, the genotypes Nistari and White Eyes Mutant, G112 and GNP, G112 and MU1all showed moderate distance and it is envisaged that they would have significant positive heterosis in cross breeding programmes (Table 4). Evaluations of traits through this cluster method indicates that these genotypes may be suitable for future crossings, maintenance of parental strains and hybridizations to maximize heterosis and to avoid inbreeding depressions.


ACKNOWLEDGMENT
The authors wish to thank Prof. G. Subramanya, Chairman, Department of Studies in Sericulture Science, University of Mysore, Manasagangotri, Mysore for providing the research facilities for this investigation.


REFERENCES

  1. Thangavelu, K., R.K. Sinha, T.S. Mahadevamurthy, S. Radhakrishnan and P. Kumaresan et al., 2000. Catalogue on Silkworm (Bombyx mori L.). Vol. 2, Central Sericultural Germplasm Resources Center, Central Silk Board, India.

  2. Nagaraju, J. and M.R. Goldsmith, 2002. Silkworm genomics-progress and prospects. Curr. Sci., 83: 415-425

  3. Sen, R., M.M. Ahsan and R.K. Datta, 1999. Induction of resistance to Bombyx mori nuclear polyhedrosis virus, into a susceptible bivoltine silkworm breed. Indian J. Sericulture, 38: 107-112.

  4. Li, M., W. Yao, Q. Hou, C.Q. Lin and K.P. Chen, 2001. Studies of some special characters in the silkworm (Bombyx mori L.) germplasm in China. Sericologia, 41: 527-542

  5. Yokoyama, T., 1963. Sericulture. A. Rv. Ent., 8: 287-306.

  6. Krishnaswami, S., 1978. New Technology of Silkworm Rearing. Central Sericultural Research and Training Institute, Mysore, India, pp: 23.

  7. SAS, SAS/ETS, 2004. SAS User’s Guide (Version 9.1). Vol. 1, SAS Institute Inc., Cary, New Jersey, USA., pp: 2170.

  8. Porto, A.J., F. Okamoto, E.A. Cunha and I.P. Otsuk, 2004. Caracterizacao de oito racas do bicho-da-seda (Bombyx mori L.). Ciencia Rural, 34: 259-264

  9. Porto, A.J. and F. Okamoto, 2003. Desempenho produtivo de quarto racas do bicho-da-seda (Bombyx mori L.) e seus cruzamentos. Bol. Ind. Anim., 60: 179-184.

  10. Rao, C.G.P., S.V. Seshagiri, C. Ramesha, B.K. Ibrahim, H. Nagaraju and Chandrashekaraiah, 2006. Evaluation of genetic potential of the polyvoltine silkworm (Bombyx mori L.) germplasm and identification of parents for breeding programme. J. Zhejiang Univ. Sci. B., 7: 215-220

  11. Petkov, N. and L. Nguyenvan, 1987. Breeding genetic studies on some lines of the silkworm, Bombyx mori L. Genetika-i- Selektsiya, 20: 384-394.

  12. Zanatta, D.B., J.P. Bravo, J.F. Barbosa, R.E.F. Munhoz and M.A. Fernandez, 2009. Evaluation of economically important traits from sixteen parental strains of the silkworm Bombyx mori L. (Lepidoptera: Bombycidae). Neotropical Entomol., 38: 327-331

  13. Mahalanobis, P.C., 1936. On the generalized distance in statistics. Proc. Nat. Inst. Sci., 2: 49-55

  14. Cruz, C.D. and A.J. Regazzi, 2001. Modelos Biometricos Aplicados Ao Melhoramento Genetico. 2nd Edn., Imprensa University, USA., pp: 390.

  15. Talebi, E., and G. Subramanya, 2009. Genetic distance and heterosis through evaluation index in the silkworm, Bombyx mori L. World Applied Sci. J., 7: 1131-1137.

  16. Arunachalam, V., A. Bandyopadhyay, S.N. Nigam and R.W. Gibbons, 1984. Heterosis in relation to genetic divergence and specific combining ability in ground nut (Arachis hypogaea L.). Euphytica, 33: 33-39.
 
 

 
Science International © 2018