Volume 6, Number 1
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Issue 1
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+ Volume 2, 2014
+ Volume 1, 2013
Science International Vol. 1 (6), 2013
Research Article
Cloning and Overexpression of a DhIFN-α1 Gene from the Halophilic Yeast of Debaryomyces hansenii into Pichia methanolica to Enhance its Tolerance to Salt and Temperature Stresses
Hsiu-Fung Chao , Rongrong Prasenmool and Yung-Fu Yen
Abstract: The interferon-α1 (IFN-α1) gene, a gene of type I interferons (IFNs), was cloned from Debaryomyces hansenii under 2.5 M of salt stress. DhIFN-α1 was constructed into a pMETB vector that carries the promoter PAUG1, which was induced by methanol. The expression cassette of PAUG1/DhIFN-α1/V5/His was excised from the vector and transformed Pichia methanolica through electroporation. The gene and expression of the transformant DhIFN-α1 was detected by PCR and dot blotting, respectively. The enhanced tolerance to salt and temperature stress of the transformant was verified by exposing it to 1.5 M of salt stress and a low and high temperature of 5 and 37C, respectively. DhIFN-α1 has been successfully cloned and expressed in P. methanolica. This study confirmed that DhIFN-α1 might enhance the tolerance of organisms to salt and temperature stress.
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