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Science International Vol. 1 (5), 2013
Research Article
Ipomoea pes-caprae Mediated Silver Nanoparticles and their Antibacterial Effect
K. Satyavani , S. Gurudeeban , T. Ramanathan and T. Balasubramanian
 
Abstract: Nanoparticle synthesis by using plant sources is a great deal of attention due to their potential in biological and pharmaceutical applications. In the present investigation, we synthesis silver nanoparticles using leaf extract of Ipomoea pes-caprae (L.) R.Br. and their antimicrobial activity against urinary tract infectious pathogens were studied. The reduction of aqueous silver nitrate ions during reaction with Ipomoea pes-caprae extract was tracked by monitoring changes in color from greenish to reddish brown. Synthesized nanoparticles were characterized by UV-Spectrophotometer, Scanning Electron Microscope and Fourier Transform Infrared spectroscopy. The pathogens collected from Urinary tract infectious patients for antimicrobial studies. UV-visible absorption spectra give a sharp peak specific at 330 nm. The synthesized silver nanoparticles were generally found to be rectangular and irregular in shape with variable size ranging from 2-45 nm as evident by SEM. FT-IR peaks were in the extract ranging from 500-4000 cm-1 which confirmed the presence of polyphenolic components on to silver nanoparticles. The silver nanoparticles showed potential antibacterial effect against urinary tract infectious microbes E. coli, P. aeruginosa, K. pneumonia, Enterobacter sp. and S. aureus. The results suggest that the application of biological synthesis silver nanoparticles against infectious bacteria provide a platform for production of broad spectrum antibiotics with cost effective and non-toxic manner.
 
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