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Science International Vol. 1 (5), 2013
Research Article
Extraction and Viability Checking of Various Carbonated Hydroxyapatite by Wharton's Jelly Mesenchymal Stem Cell
Abbas Fazel Anvari-Yazdi , Arash Yazdani , Tahereh Talaei-Khozani and Mahdi Kalantar
 
Abstract: In this study, new approaches are used to culture Wharton’s Jelly Mesenchymal Stem Cells (WJ-MSCs) on 2-Dimensional carbonated hydroxyapatite (CHAp) scaffolds extracted from bio-waste of bovine bones. To extract CHAp bioceramics, three different methods have been applied to achieve pure natural CHAp: Thermal Calcination (at temperature of 700°C), alkaline hydrothermal (275°C) and aqueous hydrothermal (250°C). Raw bovine bone and obtained apatites have been characterized by X-ray Diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). The FT-IR spectrums indicated that all of the utilized processes have the ability of plucking collagen and other organic compounds out of bovine bones and finally leaving pure natural CHAp with yield of ~60%. Fluorescent Microscopy and Scanning Electron Microscopy (SEM) were used to evaluate WJ-MSCs growth behavior, Viability and adhesion of Cells on the surface of 2-D hydroxyapatite scaffolds derived by these three different methods. In these processes, obtained apatites leave carbonate contents in specific temperatures which leads to increased biological effects in derived CHAp and preferable for orthopedic and medical usages. The findings suggest that WJ-MSCs could survive and attach onto these scaffolds and demonstrate no negative response to carbonated hydroxyapatite.
 
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